Joslin Diabetes Center

  • Research Assistant (Islet Cell and Regenerative Biology)

    Location US-MA-Boston
    Posted Date 2 weeks ago(4/9/2018 12:58 PM)
    Job ID
    R3057-18
    # Positions
    1
    Category
    Research - Research
  • Overview

    The Loeken laboratory (https://joslinresearch.org/investigators/Mary-Loeken) is searching for a Research Assistant. The research focus of the lab is the study of how maternal diabetes complicates embryonic and fetal development. The  Research Assistant provides lab support and management, assists in training new personnel, and carries out experimental procedures to contribute to lab productivity.

    Responsibilities

    • Maintain animal colonies and perform routine animal procedures, including breeding mice, dating the onset of pregnancies by presence of copulation plugs, drawing blood, injections (intraperitoneal, subcutaneous, or intravenous), minor surgical procedures, administration of inhaled or injected anesthesia, and recovery and dissection of embryos at the blastocyst stage or E 7.5-19.5.
    • Genotype offspring or embryos of several mouse strains using PCR.
    • Contribute to derivation and culture of iPSC lines using conventional and novel procedures.
    • Biochemical, histological, or molecular analysis of dissected animal tissues, cultured cells, or human samples, including:
      • Enzyme assays
      • Metabolite assays (using commercial kits or Seahorse XF analyzer)
      • Protein (Bradford) assays
      • Immunohistochemistry
      • Light and UV fluorescence microscopy
      • Immunoprecipitation and Western blot
      • RT-PCR
    • Maintain cell culture lines (especially mouse embryonic stem cells, or mouse or human induced pluripotent stem cells) and perform experiments using transfection, differentiation, and analyses of gene and protein expression.
    • Handle ordering of supplies and mice as needed, log delivery of supplies and store supplies appropriately upon delivery.
    • Order and connect gas tanks for tissue culture incubators and arrange for removal of empty tanks.
    • Monitor and enforce lab safety procedures.
    • Assist in preparation of laboratory reagents and solutions.
    • Arrange for equipment repair or maintenance.
    • Maintain lab plasmid, antibody, cell line, and animal databases.
    • Orient new fellows and students including computer access and basic laboratory procedures.
    • Maintain organized and accurate documentation of experimental procedures using electronic laboratory notebook or paper notebook as appropriate for procedures.
    • Be available for weekend work depending on cell culture or animal procedure schedules.

    Qualifications

     

    • Bachelor's degree in Biology Bichemistry, or related field. 
    • Previous undergraduate or postgraduate experience relevant to essential responsibilities of the job. 
    • Excellent data and notebook management
    • Troubleshooting of protocols and equipment
    • Strong organizational and communication skills (verbal and written)
    • Strong work ethic
    • Ability to multitask
    • General maintenance and upkeep of lab and equipment
    • Knowledge of biochemistry, cell bioloy, genetics, and mammalian embryology

     

    Techniques:

    • Histology: tissue prep, staining, microscopic analysis and imaging
    • Microscopy including bright field and fluorescent
    • Immunohistochemistry
    • Animal husbandry and colony management of mice
    • Embryo or tissue dissection using jeweler’s forceps and a dissecting microscope
    • Cell culture, especially mouse embryonic stem cell or mouse or human induced pluripotent stem cell lines
    • Molecular biology: RNA and DNA isolation, PCR, qRT-PCR, electrophoresis, gel imaging
    • Plasmid preparation  (mini- and maxi-preps)
    • Genotyping by PCR or Southern blot
    • Glucose monitoring of mice
    • Injection and minor surgical procedures of mice
    • Protein (Bradford) assays
    • Immunoprecipitation and Western blot
    • Enzyme assays (colorometric or spectrophotometric)

     

    Joslin Diabetes Center is an Equal Opportunity Employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status or any other status protected by law.

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