The Kulkarni laboratory studies the biology and function of pancreatic islets including insulin secreting beta cells and glucagon-secreting alpha cells. The lab has recently started to work on adult tissue-specific stem cells, particularly those stem cells that form the pancreatic islet cells. Current topics under investigation include generation of induced pluripotent stem cells from mouse models of type 1 and type 2 diabetes and humans with type 1 and type 2 diabetes. The studies are focused on using peripheral tissues (e.g. skin fibroblasts, blood cells and adult skeletal or adipose tissues) to generate induced pluripotent stem (iPS) cells. Future studies will be focused on generating differentiated cells from these iPS cells, including pancreatic islet cells, mature skeletal muscle and adipocytes.
A Senior Research Assistant is wanted to assist with ongoing experiments that include cell culture, molecular biology techniques, flow cytometry, histology, mammalian cell culture and animal studies, and banking of human and mouse iPS cells. The Senior Research Assistant’s responsibilities also will include maintenance of mouse colonies and genotyping of mouse strains. Some studies will involve collaboration with other investigators, and may require some assistance and work in these collaborators’ labs.
DISTRIBUTION OF TIME: The following is an approximate distribution of time that can change, based on the PI’s determination, depending on the results of experiments at different times of any given study.
Joslin Diabetes Center is an Equal Opportunity Employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status or any other status protected by law.